12 H2O+ 6 CO2 → 6 H2O+ C6H12O6 + 6 O2 9 2. Tatina, Robert E. 1986. When completing this experiment there was one mane issue with our experiment that might have affected the results. Tear up the spinach leaf and place a small number of spinach leaf fragments in the mortar, then add 2 ml of the solvent (60% isopropanol and 40% acetone) to the mortar. Photosynthesis I: An Assay Utilizing Leaf Disks. Light dependent reaction requires light energy to split water molecules (photolysis) into hydrogen ions (H^+), oxygen (O_2) and electrons to make ATP and the light dependent reaction allows plants to grow and make a waste product (oxygen), energy conversion reactions, convert solar energy to chemical energy. The leaves producing ATP, to reduce NADP to NADPH, and integrate CO2 to non-chemical molecular during process called carbon fixation. The variation of the concentration of sodium bicarbonate will have an effect on the rate of photosynthesis of spinach (Spinacia oleracea). By Cheryl G11 It comes from a leaf, winter annual plant, which evolved in the Fertile Crescent of the Middle East. Plan your 60-minute lesson in Science or Photosynthesis with helpful tips from Mitchell Smith For comparison purposes, each lab group that does this procedure should report the time at which half (5) of the disks is floating. Photosynthesis in Spinach Leaves and Respiration in Beans. This resulted in the reaction yield being 31.5%. Sunlight energy So what does the data say? Since the photosynthesis takes place in chloroplasts that has chlorophyll, the rate of photosynthesis can be affected by different colours of light, since the absorption of light changes within different colours. Print and Web Resources: ! This causes an absorption of energy from red and blue wavelengths which is used in photosynthesis. temperatures and light intensity upon the photosynthetic rate of spinach leaves were observed. It involves carbon dioxide added to water added to sun energy then is yielded to sugar and water. Purpose (photosynthesis uses light energy to convert carbon dioxide into a carbohydrate) This is the experiment because the one of the two tested cup with have baking soda in it which is Sodium Bicarbonate, which will produce Carbon Dioxide, which is need for photosynthesis. The purpose of this experiment is to determine the effect the carbon dioxide in baking soda will have on the rate of photosynthesis in spinach when placed in a de-oxygenated environment (underwater). Hypothesis 1: I believe that the spinach leaves that are only exposed to room light will have a slower rate of photosynthesis, and the spinach plants that are exposed to incandescent light will photosynthesis faster. Watch Queue Queue. The reaction requires light energy in order for it to be absorbed by chlorophyll. Photosynthesis is a process used by plants and specific types of bacteria in order to make their own food source from sunlight to chemical energy. The spinach extract has a wider range of colors than the green filter. Spinach Photosynthesis Lab Report - Google Docs. Assignment for the Leaf Disk Assay Lab. Watch Queue Queue It occurs within each chloroplast in most of the green plants, and Carbon dioxide is one of the main components of this process. The rate of photosynthesis was measured every five min under light colors of white, green, red, blue and yellow under a light intensity of 2000 lux. Preview text. Hypothesis As the concentration of carbon dioxide is increased, the rate of photosynthesis is going to increase until it reaches a certain point from which the rate of photosynthesis will be constant. When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose. It is mainly occurred within the leaves of the plant. IB biology higher level Photosynthesis : the lab - discussion & conclusion. Hydrogen peroxide is harmful to plant cells in the leaf including the chloroplast. Photosynthesis can be easily modeled and investigated through the use of spinach leaf disks, carbonated water, and light. In this experiment, the leaves will only rise to the surface when enough CO2 and light is present to allow the leaf disks to carry out photosynthesis and produce O2. When Egeria Densa leaves are kept in water and through the process of the, It usually takes place in the leaf and it is used by plants to create sugar from sunlight. Introduction & Background Spinach leaves vibrate to kick off photosynthesis July 16th, 2014 Posted by Nicole Casal Moore-Michigan It takes about one-third of a second to blink your eye. Spinach Disk Photosynthesis Lab The light source provides light energy, the solution provides water, and sodium bicarbonate provides dissolved CO 2. METHODS Pigment samples were extracted from spinach leaves by grinding and filtering the spinach leaves taken and then suspending the pigments in … Date: January Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format.All blue shaded areas require answers. Leaves usually contain CO2 and O2 and The main issue that was faced was when the leaf disks were being exposed to the higher percentages of sodium bicarbonate while they were being vacuumed in the syringe. For the treatment groups different concentrations of sodium bicarbonate were added to the distilled water to change the conditions for the leaf discs. 1985. measuring how long it took spinach leaf disks to float. We then washed the nylon off with distilled water, in order to clean it from the chemical residue, and dried it, in order to remove excess water. In the leaf-disk assay, all of the components necessary for photosynthesis are present. Without the sunlight this process and its stages wouldn’t be able to function properly to create sugar due to it being a light-dependent reaction. H2O = water Introduction: This data support the hypothesis that the cup of leafs with the Sodium Bicarbonate will have the greater amount of leafs to rise to the surface. Later, the hydrolyzed samples were allowed to cool. Light Intensity’s Effect on the Rate of Photosynthesis Measured by Light Distance and Spinach Leaf Discs Abstract In the following lab, an experiment was conducted to test the effect of light intensity (measured by distance) on the rate of photosynthesis by exposing spinach leaf discs in a sodium bicarbonate-dish soap solution to a bare lamp … Photosynthesis involves two different processes, which are light dependent reaction and light independent reaction. The plant’s chloroplasts, along with water, carbon dioxide, and light are the essential elements required for photosynthesis to take place. Taking place within plants and some bacteria, more specifically the chloroplasts. The hypothesis being tested is, If the spinach is placed in the CO2 solution, the light source will cause photosynthesis to occur and the leaves to rise. According to our data, the solution of water + sodium bicarbonate has caused the spinach leaves to begin respiration of photosynthesize causing them to rise in the presence of carbon dioxide. There are two types of photosynthetic processes; one includes oxygenic photosynthesis, which is most common and demonstrated through plants, algae and cyanobacteria while the other one is an-oxygenic photosynthesis. Through this experiment, we want. Using the floating disk technique in our experiment to measure the rate of photosynthesis, spinach leaves will be submerged in a concentration of CO2 and a concentration of water. This is a process which happens when the sun (and certain types of lights) is present. Faster Rates of Photosynthesis in Spinach ( Spinacia Oleracea ) Leaves as Light Intensity SurgesAbstractPhotosynthesis is a food making process that autotrophic plants use. We kept wrapping nylon pieces around the glass rod with the tweezers until the nylon became white and was not clear anymore. C. Errors of this lab include improper deflation of the spinach leaves, improper ratio of soap and water, correct amount of baking soda. photosynthesis, and some possible assumptions that can be drawn from the correlation of the data gathered. Background information: Spinach was used during this experiment and is a member of the family of ‘Chenopodiaceae’. 2 However, is it possible to count in the colour of light, too, which I am going to investigate in this lab. ... the spinach was punched and many leaf disks were made. might measure the gross rate of photosynthesis with this technique? Title: This shows how plants like spinach can hold there produce oxygen in the leafs which gives them there floating properties. The American Biology Teacher, 47(2):96-99. Steucek, Guy L. Robert J. Hill and Class/Summer 1982. Purpose: The purpose of this experiment was to determine the effect of sodium bicarbonate on the rate of photosynthesis of spinach leaf disks. Investigation of the effects of light intensity on Egeria Densa’s leaves photosynthesis in the observation on leaf After recording the rates of photosynthesis, the mean was calculated by taking the rates measured and adding them together, then dividing by the number of replicants to find the average between them all. In this lab, a dye reduction technique was used. Photosynthesis lab report. Simon Fraser University. The “photo” part of photosynthesis is in reference to the fact that the process requires light. Student name: Eren Kuriyama This preview shows page 1 - 2 out of 5 pages. View full document. spinach leaves are placed under blue or red light, the rate of photosynthesis will be faster than green light because chlorophyll a and b as well as several types of carotenoids are present in the spinach. Both the spinach extract and the green filter are green in color. General Biology (BISC 102) Academic year. C6H12O6 = glucose When exposed to light, the disks use carbon dioxide and water to produce oxygen and glucose. Photosynthesis is the process by which plants and other photoautotrophs synthesize organic compounds from carbon dioxide (Faculty of Science and Horticulture, 2018). Internal Assignment The overall balanced equation is: Investigation of the effects of light intensity on Egeria Densa’s leaves photosynthesis in the observation on leaf In this experimentwe analyzed that rate at which photosynthesis occurs by observing the time it took for Spinach( S. … Glycolate is first oxidised to a glyoxylate molecule yielding hydrogen peroxide as aby-product. In this experiment it was ultimately found that the 95% confidence interval bars of treatment group 1 and treatment group 2 overlap, however none of the treatment groups overlap with each other or the control. The first part of this lab was making the bicarbonate and soap solution. In testing the hypothesis we use 4 test experiments 1 with soapy water as the negative control, one with Sodium bicarbonate as the positive control, one with no light, and one with warm water and light. Then the discs were timed on how long it took for half of the fifteen discs to float to the surface. Fifteen leaf discs were placed in a syringe with water and using the syringe the air was vacuumed out of the leaf discs. This experiment is set up the study the effect of different sodium bicarbonate concentrations on the rate of photosynthesis in spinach (Spinacia oleracea). This experiment deals with measuring the amount of oxygen accumulated. 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